I think so. I have several older Myst games running my Vista 32. Ages Beyond Myst currently. I don't remember if I've run Myst since I changed to Vista.
You can get help from the Myst community at Myst Online Uru Live... MOUL.
Other places you can try are Uru Obsession and the Guild of Writers
The communities were very active. Since the close of Myst Online in April 2008 activity has been decreasing. The game was to go open source this Spring. That has yet to happen. We are still hoping for later this year. If so thing will revive.
There is a large contingent of Myst fans in Second Life. Look for the group D'ni Refugees to connect. If you are a Myst fan and have not visited Second Life check out the Second Life Help for Explorers on the Guild of Maintainers' site. Many of the areas of Ages Beyond Myst have recreated in SL. Not the same, but if you played ABM they bring back good memories.