Width of these vertical lines are about 1/8 of width of an entire sprite. It indicates that the problem could be associated with one bit of data only. For instance - a load of the one cartridge data line. That's why I asked for a floppy. The fact that the game works well with the original GTIA may or may not prove damage of the Sophia 2. Threshold voltages of the Sophia 2 and GTIA inputs are different. A short movie is not enough to determine the cause. If I had a possibility to put the Sophia into another computer, I would try this. A lot of information could also be provided by the Atari Control Picture acp.exe but since you don't have a SIO2PC...